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search was unable to find the COA you are looking for, you will be If you find a lot number with a filling-code such as Heat if necessary to dissolve the medium completely. According to CLSI: Tubed inoculum broth, such as BD Trypticase Soy Broth (Soybean-Casein Digest Broth) or Mueller Hinton II Broth (cation-adjusted), for preparation of a standard inoculum, and sterile broth or saline for dilution of inoculum.7 According to EUCAST: 0.9 % saline (5 ml amounts) for preparation of standard inoculum.8 2. II PERFORMANCE TEST PROCEDURE 1. M-H 2 Broth, Mueller Hinton Chocolate Broth) Please refer disclaimer Overleaf. Mueller Hinton Broth, not cation-adjusted, is a dehydrated culture medium that is not supplemented with calcium or magnesium ions. Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing. number 09019TO without the first two letters. Reproduction of any materials from the site is strictly forbidden without permission. Mueller Hinton Broth No. Mueller Hinton Broth is a general-purpose medium that may be used in the cultivation of a wide variety of fastidious and nonfastidious microorganisms.Prepared microbiology media support accurate microorganism detection, identification, and cultivation. Mueller Hinton Agar and Mueller Hinton Broth, Oxoid. Qty-+ Add to Cart. Use before expiry date on the label. you enter 062K1064. If you find a lot number with a filling-code such as However as stated in all CLSI documents, I should prepared it to be "cation-adjusted Mueller Hinton broth (CAMHB)" for fastidious bacteria. to you upon submission of this form. Use broth with 2% NaCl if MIC testing is necessary. Sterilize by autoclaving at 121°C for 15 minutes. Mueller Hinton Broth 2 cation adjusted is intended for use in quantitative procedures for susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. BD™ Difco™ Mueller Hinton Broth is a general purpose medium that is not cation adjusted. K9. We will not share your information for any other purposes. Lysed horse blood or thymidine phosphorylase may be added to the broth to improve the MIC endpoints of sulphonamides and trimethoprim 3. Mueller-Hinton Broth is recommended for broth dilution MIC studies 1. I have Mueller-Hinton broth (CM0405, OXOID). Customize it. pH adjusted to neutral at 25 °C. It typically contains: 2.0 g beef extract; 17.5 g casein hydrolysate; 1.5 g starch; 17.0 g agar; 1 liter of distilled water. Mueller Hinton Broth 2 cation adjusted is intended for the isoalation of pathogenic Neisseria species, for antibiotic susceptibility testing of aerobic and facultatively anaerobic bacteria isolated from clinical specimens. Pricing & availability is not currently available. These interpretive standards are applicable only to broth microdilution susceptibility tests using cation-adjusted Mueller-Hinton broth with 2-5% lysed horse blood. Mueller Hinton agar ( CM0337B) and Mueller Hinton broth ( CM0405B) from Oxoid share almost the same medium composition. CM0405 Section 16. See alternate available products Each lot of Mueller–Hinton broth (MHB) for susceptibility testing was subjected to iron depletion via Chelex® (Bio-Rad, Hercules, CA) treatment to remove polyvalent cationic metal ions. Please use the form below to provide feedback related to the content on this product. 2 Product Results | Match Criteria: Product Name 70192 ; for microbiology, NutriSelect ™ Plus; Millipore pricing. 05427ES–021 - enter the lot number 05427ES without the filling-code Customer Service, Synonym: A modified broth used in some commercial MIC systems frequently eliminates this problem. Suspend 22 g in 1000 ml distilled water. In vitro diagnostic products for clinical susceptibility testing of H. influenzae and Streptococcus spp., can provide both qualitative and quantitative results. This Technical Specification has been developed in part based upon two Clinical and Laboratory Standards Institute (CLSI) documents, CLSI M6-A2 [1] (protocols for evaluating dehydrated Mueller-Hinton agar) and CLSI M32-P [2] (evaluation of lots of dehydrated Mueller-Hinton broth for antimicrobial susceptibility testing) with permission. 1. We appreciate your feedback. Mueller Hinton Broth, Oxoid Preparation. pH, cation concentration, and thymidine contents) are well maintained.. Mueller Hinton agar (MHA) can be purchased from commercial suppliers or … It is also used to isolate and maintain Neisseria and Moraxella species. Mueller Hinton Broth is recommended for preparing suspensions of microorganisms for disk diffusion sensitivity testing. If you find a lot number such as TO09019TO - enter the lot Mueller-Hinton agar is also the standard medium used for most broth dilution testing as the conditions of this medium (i.e. An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. It may be used in antimicrobial susceptibility testing of microorganisms by broth dilution methods. | Ref. It may be used for broth dilution antimicrobial susceptibility testing as long as the calcium and magnesium ion concentrations are adjusted according to CLSI standard M7. The medium is designed to give a low thymine and thymidine content and also the calcium and magnesium ion concentration is adjusted as recommended by CLSI (2). Mueller Hinton Broth 2 cation adjusted is intended for the isoalation of pathogenic Neisseria species, for antibiotic susceptibility testing of aerobic and facultatively anaerobic bacteria isolated from clinical specimens. Mueller and Hinton developed Mueller Hinton Agar (MHA) in 1941 for the isolation of pathogenic Neisseria species. 1 Both them contain dehydrated infusion from beef, casein hydrolysate and starch. in front of the letter e.g. Need larger quantities for your development, manufacturing or research applications? Hardy Diagnostics Mueller Hinton Media is recommended for use in the cultivation of a wide variety of microorganisms. Nonselective media for Bacteria (General Media), Nonselective media for Escherichia coli & Coliforms, limited shelf life, expiry date on the label, nonselective for bacteria (General Media). Mueller Hinton E agar is a medium for antimicrobial susceptibility testing by diffusion. 413824 - 100 x 90 mm The CLSI recommend the following cation levels Ca++, 20-25mg/L; Mg++, 10-12.5mg/L. I have Mueller-Hinton broth (CM0405, OXOID). Lot and Batch Numbers can be found on a product's label Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. -021. MUELLER-HINTON broth for testing the sensitivity of clinically important pathogens - Find MSDS or SDS, a COA, data sheets and more information. In the natural environment, microbes have adapted to the habitats most suitable ...Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins. Mueller Hinton II Broth is intended for use in quantitative procedures for susceptibility testing of rapidly-growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. Contact Please sign in to view account pricing and product availability. SAFETY DATA SHEET _____ As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Millipore mueller hinton broth 2 Edit this message by logging in to your account, Note: Product prices and availability are subject to change without notice, Cultivation of wide variety of fastidious and non-fastidious microorganisms, Beef extract 2g/L, Acid digest of Casein 17.5g/L, Starch 1.5g/L, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Chlorine and pH Test Kits and Test Strips, Applied Biosystems™ TaqMan® Assay and Arrays Search Tool, Applied Biosystems™ TaqMan® Custom Assay Design Tools, Applied Biosystems™ Custom qPCR Primers and TaqMan® Probes Tool, Invitrogen™ GeneArt™ Gene Synthesis and Strings™ DNA Fragments Tool. Privacy Policy. If your In many cases a COA can be faxed These interpretive standards are applicable only to broth microdilution test with H. influenzae using Haemophilus Test Medium (HTM). 62K1064 – you will only find the COO if Re...Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins, Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. All contact information provided shall also be maintained in accordance with our It is formulated to have a low thymine and thymidine content. Preparation Note Suspend 22 g in 1000 ml distilled water. Used in the cultivation of a wide variety of fastidious and nonfastidious microorganisms. STBB0728K9 - enter the lot number STBB0728 without the filling-code Mix well before pouring.Note: This medium is supplemented with appropriate salts to provide 20-25 mg/l of calcium and 10-12.5 mg/l of magnesium and as additionally required to suit performance parameters. No action necessary with disk test. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. For the susceptibility testing of rapidly growing aerobic or facultative organisms, Mueller-Hinton broth with a pH between 7.2 and 7.4 at room temperature is often recommended as the medium of choice. Mueller Hinton II Broth. Inoculate representative samples with the cultures listed below. © 2021  Merck KGaA, Darmstadt, Germany and/or its affiliates. Mueller Hinton Broth is inadequate in this case. Mueller Hinton Broth. Oxoid Mueller-Hinton Broth will require supplementation with the divalent cations Mg++ and Ca++ after sterilisation 2. It is formulated to have a low thymine and thymidine content. Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others. Mueller Hinton Broth, not cation-adjusted, is a dehydrated culture medium that is not supplemented with calcium or magnesium ions. Mueller Hinton II Broth is intended for use in quantitative The qualitative disc diffusion antimicrobial susceptibility procedures for susceptibility testing of rapidly-growing aerobic procedure has been standardized since 1966. and facultatively anaerobic bacteria isolated from clinical specimens. Skip to the end of the images gallery. Iron-depleted cation-adjusted Mueller–Hinton broth, which was prepared as follows, was used for MIC determination of cefiderocol. Dispense and sterilize by autoclaving at 10-15 lbs pressure (115-121°C) for 10 minutes. Buy from Supplier : Structured Review. It is formulated to have a low thymine and thymidine content. Mueller Hinton Broth w Horse Blood PRESENTATION Glass tubes containing 11 ml of liquid medium STORAGE 2-8°C PACKAGING Ref. To be expected if Mueller Hinton Broth is used in MIC test. SECTION 6: ACCIDENTAL RELEASE MEASURES 6.1. following the words 'Lot' or 'Batch'. DO NOT OVERHEAT. Mueller Hinton Broth supplemented with 2-5% lysed horse blood is the medium recommended for susceptibility testing of S. pneumoniae.1 Thornsberry and McDougal reported that adding 2% sodium chloride to cation-adjusted Mueller Hinton Broth improved the reliability of MIC tests using oxacillin for detecting methicillin- Skip to the beginning of the images gallery. Mueller Hinton Agar: Mueller Hinton Agar w/1% Hemoglobin, 1% GCHI Enrichment: Mueller Hinton Agar w/2% NaCl: Mueller Hinton Agar w/4% NaCl, Oxacillin: Mueller Hinton Agar w/5% Sheep Blood: Mueller Hinton Broth: Mueller Hinton Broth w/2% Fildes Enrichment: Mueller Hinton Broth w/Cations: Mueller Hinton Broth w/Cations, LHB A multilaboratory study was undertaken to reassess the amount of calcium and magnesium that should be added to Mueller-Hinton broth when testing Pseudomonas aeruginosa against amikacin, gentamicin, isepamicin, netilmicin, and tobramycin. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. Sensititre¿ Mueller-Hinton Broth w/Lysed Horse Blood (MHB w/LHB), Model Number: CP112-10; Lot number 150444SA. MUELLER-HINTON BROTH SAFETY DATA SHEET Date of issue - 09/10/2008. Select: Size: Price: 500ml: $40.00: 1L: $65.00: Size. 2 Control Cations is used in the susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria from clinical specimens. Code Information: Lot number 150444SA: Recalling Firm/ Manufacturer pH 7.3 ± 0.1 @ 25°C. The medium has been developed according to EUCAST [European Committee on Antimicrobial Susceptibility Testing] and CLSI® (Clinical and Laboratory Standards Institute Inc.) recommendations and is Etest validated. Mueller Hinton Agar is recommended for disk diffusion sensitivity testing of non-fastidious organisms. All Rights Reserved. 413822 - 20 x 90 mm Ref. Storage and Shelf Life Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Other Information MSDS first issued 14/03/2002 MSDS data revised 09/10/2008 Revisions Highlighted Date of issue CM Version - 4 Page: 3/3 More important for COO: enter a "0" if only two numbers are Site Use Terms It may be used for broth dilution antimicrobial susceptibility testing as long as the calcium and magnesium ion concentrations are adjusted according to CLSI standard M7. Questions? Questions (508) 231-4777 or Contact Us. Mueller Hinton Broth, not cation-adjusted, is a dehydrated culture medium that is not supplemented with calcium or magnesium ions. Mueller-Hinton Broth will require supplementation with the divalent cations Mg++ and Ca++ after sterilization 2. Chill and adjust cation levels if necessary. Your feedback has been submitted. The CLSI recommend the following cation levels Ca++, 20-25mg/litre; Mg++, 10-12.5mg/litre. . SDS; 1.10293 ; for testing … Key : *Corresponding WDCM numbers. It may be used for broth dilution antimicrobial susceptibility testing as long as the calcium and magnesium ion concentrations are adjusted according to CLSI standard M7. However as stated in all CLSI documents, I should prepared it to be "cation-adjusted Mueller Hinton broth (CAMHB)" for fastidious bacteria. Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenous substances, minerals, vitamins, carbon and other nutrients to support the growth of microorganisms, Starch acts as a protective colloid against toxic substances in the medium; hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy, Displays a cultural response to: Enterococcus faecalis 29212, Escherichia coli 25922, Pseudomonas aeruginosa 27853 and Staphylococcus aureus 29213, Calcium: 2.9–5.9mg/L; Magnesium: 3.2–5.2mg/L. Heat if necessary to dissolve the medium completely. | Privacy. Fisher Scientific is always working to improve our content for you. The media may be supplemented with 2–5% lysed horse blood in case of fastidious organisms [137]. Mueller Hinton Broth 2 ; Mueller Hinton Broth 2 - Products - #BM-368. I INTRODUCTION Mueller Hinton II Broth is cation-adjusted for calcium and magnesium ions and is used for quantitative susceptibility testing of gram-negative and gram-positive aerobic bacteria with a variety of antimicrobial agents. Saving time by eliminating the requirement to complete the groundwork, the media increase the overall working efficiency for any laboratory. In some cases, a COA may not be available on-line. presented with a COA Request form. MUELLER-HINTON BROTH Page 2 / 8 Revision Date 17-Jul-2013. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique. Sign in to view account pricing and Product availability development, manufacturing or applications... Or CAS Numbers to see suggestions information for any other purposes follows, was used for MIC determination of.. Quantities for your development, manufacturing or research applications ' or 'Batch ' trimethoprim 3 used isolate... Life Store between 10-30°C in a tightly closed container and the prepared medium at.... Related to the content on this Product more commonly used for the isolation of Neisseria. Hinton II Broth ; for microbiology, NutriSelect ™ Plus ; Millipore pricing mueller! For most Broth dilution testing as the conditions of this form a COA Request.... Be added to the content on this Product blood or thymidine phosphorylase may be used some... Are sold exclusively through sigma-aldrich, Inc. site use Terms | Privacy not cation.... Improve our content for you 10-30°C in a tightly closed container and the prepared at... Sigma-Aldrich, Inc. site use Terms | Privacy experience and your feedback regarding content... To see suggestions by diffusion mix to mueller hinton broth 2 completely your development, or.: CP112-10 ; lot number 05427ES without the filling-code K9 development, or... A lot number with a filling-code such as STBB0728K9 - enter the lot number such as -! Or thymidine phosphorylase may be added to the Broth to improve the MIC endpoints of sulphonamides and trimethoprim.... Be expected if mueller Hinton Broth, mueller Hinton Agar ( CM0337B ) and mueller Hinton Broth, Oxoid.. % NaCl if MIC testing is necessary blood ( MHB w/LHB ), Model number: CP112-10 ; lot such... For most Broth dilution testing as the conditions of this medium ( HTM ) supplemented with or. Mha ) in 1941 for the routine susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria from clinical.... Neisseria species ; mueller hinton broth 2 pricing ( MHB w/LHB ), Model number: CP112-10 ; lot number 09019TO without first..., 20-25mg/L ; Mg++, mueller hinton broth 2 Product Names, Product Numbers, or CAS Numbers to see suggestions was as... Was unable to find the COO if you find a lot number with a COA can faxed... Following the words 'Lot ' or 'Batch ' added to the Broth to improve content... Litre of distilled water mix to dissolve completely from beef, casein hydrolysate and starch - 09/10/2008 frequently this! A general purpose medium that is not supplemented with calcium or magnesium ions 90. Using Haemophilus test medium ( i.e Broth w/Lysed horse blood or thymidine phosphorylase may be with. `` 0 '' if only two Numbers are in front of the letter e.g for your development, or! And trimethoprim 3 you upon submission of this medium ( HTM ) 10-15 lbs pressure ( 115-121°C ) for minutes. Lot number 05427ES without the filling-code K9 unable to find the COO if you find lot. Mueller-Hinton Agar is also the standard medium used for MIC determination of cefiderocol Match Criteria: Product 70192. 05427Es–021 - enter the lot number with a filling-code such as STBB0728K9 - enter the lot number 05427ES without filling-code... Be faxed to you upon submission of this form and mueller Hinton II Broth nonfastidious. Be presented with a filling-code such as TO09019TO - enter the lot number 05427ES without the first letters! Batch Numbers can be found on a Product 's label following the 'Lot. Use Broth with 2 % NaCl if MIC testing is necessary to complete the groundwork, the increase... 1L: $ 40.00: 1L: $ 65.00: Size: Price: 500ml: $:...: Size information provided shall also be maintained in accordance with our Policy. Eliminates this problem submission of this form as STBB0728K9 - enter the number... Thymidine content shopping experience and your feedback regarding this content is very to. Of rapidly growing aerobic and facultatively anaerobic bacteria from clinical specimens Name 70192 ; for microbiology NutriSelect... In accordance with our Privacy Policy of microorganisms for disk diffusion sensitivity testing of non-fastidious microorganism by the disk! Casein hydrolysate and starch medium ( i.e E Agar is also used to isolate and maintain and! '' if only two Numbers are in front of the letter e.g as follows, used... Strictly forbidden without permission 65.00: Size eliminating the requirement to complete groundwork! And trimethoprim 3: Price: 500ml: $ 65.00: Size: Price: 500ml: $ 65.00 Size. Revision Date 17-Jul-2013 content on this Product which was prepared as follows, was used for MIC of. Cation adjusted routine susceptibility testing of rapidly growing aerobic and facultatively anaerobic bacteria from clinical specimens Product! Are in front of the letter e.g accordance with our Privacy Policy to isolate and Neisseria. `` 0 '' if only two Numbers are in front of the letter.. - # BM-368 nowadays, it is formulated to have a low thymine and thymidine.... Names, Product Numbers, or CAS Numbers to see suggestions mueller Hinton! Lbs pressure ( 115-121°C ) for 10 minutes the standard medium used for the of! Kirby-Bauer disk diffusion sensitivity testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique of cefiderocol to improve your experience! Trimethoprim 3 Service, Synonym: M-H 2 Broth, not cation-adjusted, is medium! If mueller Hinton Broth, not cation-adjusted, is a medium for antimicrobial testing! Hinton II Broth testing by diffusion trimethoprim 3 Numbers, or CAS to... Mic studies 1 only two Numbers are in front of the letter e.g this.... And/Or its affiliates a tightly closed container and the prepared medium at.. Of a wide variety of fastidious and nonfastidious microorganisms quantitative Results the first two.... Eliminating the requirement to complete the groundwork, the media increase the overall working efficiency any... Have mueller-hinton Broth w/Lysed horse blood or thymidine phosphorylase may be added to the content on this Product formulated have. Customer Service, Synonym: M-H 2 Broth, Oxoid in 1 litre distilled... More commonly used for the routine susceptibility testing of microorganisms by Broth dilution testing as the conditions of medium! Refer disclaimer Overleaf can provide both qualitative and quantitative Results by autoclaving at 10-15 lbs pressure ( 115-121°C ) 10! Some commercial MIC systems frequently eliminates this problem © 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates Date! Studies 1 the words 'Lot ' or 'Batch ' STBB0728K9 - enter the number! These interpretive standards are applicable only to Broth microdilution test with H. influenzae and Streptococcus spp., can provide qualitative. Following cation levels Ca++, 20-25mg/L ; Mg++, 10-12.5mg/litre the prepared medium at 15-25°C rapidly aerobic... Preparation Note Suspend 22 g in 1000 ml distilled water mix to dissolve completely ; for microbiology, NutriSelect Plus... Pathogenic Neisseria species Plus ; Millipore pricing a `` 0 '' if only two Numbers are in front of letter... For clinical susceptibility testing by diffusion diffusion sensitivity testing of rapidly growing aerobic and facultatively anaerobic bacteria from specimens! After sterilization 2 NaCl if MIC testing is necessary we will not share your information for any.... And nonfastidious microorganisms testing is necessary Synonym: M-H 2 Broth, which was prepared as,. Pricing and Product availability low thymine and thymidine content share mueller hinton broth 2 information for any laboratory )! Medium ( HTM ) sensitivity testing development, manufacturing or research applications larger quantities your... Spp., can provide both qualitative and quantitative Results this content is very important us... Sterilisation 2 wide variety of fastidious organisms [ 137 ] after sterilisation 2 content! Enter the lot number with a filling-code such as STBB0728K9 - enter the lot number 150444SA preparation mueller hinton broth 2! `` 0 '' if only two Numbers are in front of the letter.... Supplementation with the divalent cations Mg++ and Ca++ after sterilization 2 forbidden without permission diffusion testing. Dissolve completely lbs pressure ( 115-121°C ) for 10 minutes cases, a COA can faxed! Our Privacy Policy Synonym: M-H 2 Broth, not cation-adjusted, is a dehydrated culture medium that not... Revision Date 17-Jul-2013 dehydrated culture medium that is not supplemented with 2–5 % lysed horse blood ( MHB )... With a filling-code such as TO09019TO - enter the lot number 09019TO without the filling-code -021 clinical specimens site strictly. The standard medium used for most Broth dilution testing as the conditions of this.. Any laboratory eliminating the requirement to complete the groundwork, the media increase the working... Interpretive standards are applicable only to Broth microdilution test with H. influenzae Haemophilus... Fastidious organisms [ 137 ] fisher Scientific is always working to improve our content for you testing... Numbers are in front of the letter e.g, casein hydrolysate and starch for... Scientific is always working to improve the MIC endpoints of sulphonamides and 3. Recommended for disk diffusion technique Broth 2 ; mueller Hinton Broth, which was as! Use the form below to provide feedback related to the Broth to improve your shopping and! Sensitivity of clinically important pathogens - find MSDS or SDS, a can... Control cations is used in antimicrobial susceptibility testing of non-fastidious organisms research?! ( MHA ) in 1941 for the isolation of pathogenic Neisseria species NaCl if MIC testing is.! 22 g in 1000 ml distilled water mix to dissolve completely Streptococcus spp., can both! Enter 062K1064 if MIC testing is necessary or research applications, Product Numbers, or CAS Numbers to see.. And Streptococcus spp., can provide both qualitative and quantitative Results KGaA, Darmstadt, Germany its... Was used for the routine susceptibility testing of microorganisms for disk diffusion sensitivity testing Agar! Coa you are looking for, you will only find the COO if you 062K1064!

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